A three–decade survey of Brazilian <i>Fusarium oxysporum</i> f. sp. <i>lycopersici</i> races assessed by pathogenicity tests on differential tomato accessions and by molecular markers

Physiological race determination of 143 Fusarium oxysporum f. sp. lycopersici (FOL) isolates collected along 30 years in major tomato‐producing regions Brazil. races were determined via root‐dipping inoculation differential tomato accessions and by the PCR‐based marker system Hirano Arie (2006). According to pathogenicity/virulence assays, five 1, 23 2 115 3 identified. FOL 1 prevailed up early 2000s. Afterwards, large majority was classified as invasive 3. Novel reports done states, thus expanding its geographical distribution. Using this system, a precise discrimination observed for all isolates. However, displayed only cosmopolitan 1–specific amplicon pattern. The development and/or validation novel race‐specific systems are necessary allow potentially endemic Brazilian 2. present characterization indicates that distinct evolutionary mechanisms acting select new genetic variants across agroecosystems around globe.